blood lab#2
| Blood Lab #2: ABO/Rh Blood Typing | ||||||
| Purpose: Blood spatter was left at a crime scene. Investigators believe the perpetrator was cut on the | ||||||
| weapon and left his/her blood at the scene.Four persons of interest may have been at the crime scene. | ||||||
| In this experiment, the technique to identify an individuals using ABO/Rh blood typing will be used to | ||||||
| determine the identity of the suspect who left his/her blood at the scene. | ||||||
| Procedure Part I: (copy the data table into your notebook) | ||||||
| 1. Using a marker, label the 5 blood typing spot plates for each individual | ||||||
| suspect as W, X, Y, and Z. Add one more plate as the Crime Scene (CS) | ||||||
| 2. Add 3 drops of Person W's blood in each of the 3 wells on the spot plate. | ||||||
| 3. Add 3 drops of anti-A Sera to the A well. | ||||||
| 4. Use a tooth pick to gently stir the 6 drops . (do not stir hard or it will scratch the plate!). | ||||||
| Throw away used tooth pick!!! | ||||||
| 5. Observe the spot plate against a white background to note any clumping. | ||||||
| 6. Record you results in the data table as "clumping" or "no result". | ||||||
| 7. Add 3 drops of anti-B Sera to the B well. | ||||||
| 8. Use a tooth pick to gently stir the 6 drops . (do not stir hard or it will scratch the plate!). | ||||||
| Throw away used tooth pick!!! | ||||||
| 9. Observe the spot plate against a white background to note any clumping. | ||||||
| 10. Record you results in the data table as "clumping" or "no result". | ||||||
| 11. Add 3 drops of anti-Rh Sera to the Rh well. | ||||||
| 12. Use a tooth pick to gently stir the 6 drops . (do not stir hard or it will scratch the plate!). | ||||||
| Throw away used tooth pick!!! | ||||||
| 13. Observe the spot plate against a white background to note any clumping. | ||||||
| 14. Record you results in the data table as "clumping" or "no result". | ||||||
| 15. REPEAT PROCEDURES 2- 14 USING PERSONS X, Y AND Z. | ||||||
| Procedure part II: IDENTIFICATION OF THE CRIME SCENE BLOOD SWATCH. | ||||||
| 1. Obtain a the crime scene bindle from the crime lab manager. | ||||||
| 2. Record your bindle number in your data table, open the bindle and remove the swatch | ||||||
| using the tweezers. | ||||||
| 3. Use a pair of scissors to cut the swatch into 4 small squares- place them back into the | ||||||
| bindle using the tweezers. | ||||||
| 4. Place 1 small square swatch into the 3 wells on the spot plate labeled CS. | ||||||
| 5. Add 3 drops of distilled water on top of the swatch. Let stand for 1 minute as the blood | ||||||
| dissolves into the water. | ||||||
| 6. REPEAT procedures 3-14 from Part I. | ||||||
| CLEAN UP : Rinse the spot plates into the wash bin using the distilled water bottles. | ||||||
| Return the dried spot plates to the blood typing kits. | ||||||
| Data Table : ABO/Rh Blood Typing | ||||||
| Anti- A Sera | Anti-B Sera | Anti- Rh Sera | blood type? | |||
| Person W | ||||||
| Person X | ||||||
| Person Y | ||||||
| Person Z | ||||||
| Bindle #___ | ||||||
| Crime Scene | ||||||
| Conclusion Questions: You must answer in complete sentences in your notebook! | ||||||
| 1. Using your results, what is the identity of the individual whose blood was found at | ||||||
| the crime scene? Which W, X, Y or Z? Explain. | ||||||
| 2. What percentage to the general population could have the same blood type | ||||||
| as your suspect? | ||||||
| 3. Describe how to conclusively determine the identity of the suspect whose blood | ||||||
| was left at crime scene. | ||||||